关键词: 乳双歧杆菌 V9, 小鼠, 免疫功能, 剂量

Abstract:

Objective Probiotics play an important role in the immune regulation of the body, and different strains often have different regulatory functions on immune cells. The present study aimed to evaluate the immunomodulatory effects of Bifidobacterium lactis V9 in mice and its mechanism. Method Normal C57BL / 6J mice were randomly divided into 4 groups ( n = 10) , and intragastrically administrated distilled deionized water, 0. 5 mg / kg, 1. 0 mg / kg, and 3. 0 mg / kg of Bifidobacterium lactis V9 for 30 days. After that, the mice were killed and the organ / body weight ratio of mice was measured. The cellular immunity was evaluated by splenic lymphocyte transformation assay and delayed hypersensitivity. The function of monocyte macrophage was evaluated by carbon clearance test and mouse peritoneal macrophage phagocytosis of chicken red blood cells. The humoral immunity of mice was evaluated by antibody-producing cell assay and serum hemolysin assay. NK cell activity was evaluated by NK cell activity assay. Result Bifidobacterium lactis V9 showed no significant effects on organ ratios of the mice compared with the negative control(P> 0. 05). Bifidobacterium lactis V9 significantly increased the transformation ability of mice splenic lymphocytes, murine macrophage carbon clearance function, mouse peritoneal macrophage phagocytosis of chicken red blood cells, hemolytic activity and NK cell activity ( P < 0. 05 ) . Conclusion Bifidobacterium lactis V9 exhibited potent to enhance the immunomodulatory effects, and provide a new idea for its further development and utilization.

Key words: Bifidobacterium lactis V9, mouse, immunomodulatory effects, dose